Nucleic acid oxidation plays an important role in the pathophysiology progress of a variety of diseases. 8-oxo-7,8-dihydro-\n2�-deoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn), which originate from DNA and RNA\noxidation, were the most widely used indicators for oxidative stress. The study investigated the relation between 8-oxodGsn,\n8-oxo-Gsn, and CKD. 146 patients with CKD were divided into five disease stages, and their fasting blood and\nmorning urine were collected. The levels of 8-oxo-dGsn and 8-oxo-Gsn in plasma and urine were quantified by LCMS/\nMS. The ratio of urinary 8-oxo-Gsn to creatinine increased from stages 1 to 4 corresponding to the increased\nseverity of CKD, but it decreased in stage 5. And plasma 8-oxo-Gsn gradually increased with the decline of renal\nfunction. In particular, the increased ratio of plasma and urine 8-oxo-Gsn in stage 5 exceeded the concentration of\ncreatinine. This trend was similar to the estimated glomerular filtration rate (eGFR), which indicates that 8-oxo-Gsn\ncould be an appropriate indicator for renal function. Our finding indicates that as the disease progresses, RNA oxidation\nis increased. The significant increase in the ratio of plasma and urinary 8-oxo-Gsn is a novel evaluation index of end-stage\nrenal disease.
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